The major components of LunaGel™ include the ECM proteins collagen type I, III, IV, and V, as well as connective tissue glycoproteins and proteoglycans. LunaGel™ retains the intrinsic cell-instructive bioactivity of natural ECMs facilitating cell attachment, proliferation, differentiation, migration, and proteolytic degradation. LunaGel™’s unique photocrosslinking technology allows unprecedented control over matrix porosity and stiffness, allowing researchers to replicate the physicochemical properties of a variety of healthy and diseased tissues in 3D cell culture applications.
LunaGel™ ECM has been successfully used in a wide range of applications including
cell attachment and proliferation
stem cell culture and differentiation
cancer spheroid assays
How LunaGel™ Photocrosslinkable Extracellular Matrix Works
LunaGel™ is supplied as a sterile solution with freeze-dried photoinitiator. Available as Low Stiffness (0 - 6.5 kPa) and High Stiffness kit (0 - 25 kPa). Reconstitute the photoinitiator in PBS, mix with LunaGel™ ECM and cells, and photocrosslink the solution using the Luna Crosslinker™ to form 3D cell culture models. By controlling the duration of light exposure you can produce hydrogels with a specified stiffness. Add your favourite culture media and you're culturing in 3D!
LunaGel™ - Porcine Skin Gelatin - Photocrosslinkable Extracellular Matrix
This kit contains enough LunaGel™ to create a total volume of 10mL (low stiffness) or 7.5mL(high stiffness) hydrogel.
- Low stiffness kit: 5mL LunaGel™ ECM (2 x solution, sterile)
- High stiffness kit: 5mL LunaGel™ ECM (1.5 x solution, sterile)
- 5 vials of photoinitiator (freeze-dried, sterile)