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Frequently Asked Questions

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Is LunaGel is made of 100% natural ingredients or mixed with any chemical components?

LunaGel contains a proprietary mixture of photocrosslinkable Gelatin and a visible light photoinitiator dissolved in phosphate buffered saline. No chemicals are added to LunaGel.


For most of the natural gelatins, they would become thermally unstable or melt when temperature is close to 37 degrees. What's the stable temperature window for your porcine gelatin lunagel after curing?

LunaGel forms hydrogels by photocrosslinking of chemically reactive groups and forms stable hydrogels. Unlike unmodified gelatins, LunaGel does not melt when heated.


How do you suggest to monitor the light source of your system? Also, in case of problems, do you have spare lamps with 405 nm? How does the maintenance of your system work?

The LEDs we use have a guaranteed lifespan with at least 95% of initial intensity of 50,000 hours (8 hours/day for 17 years) and the device is basically maintenance free.


Can we use black walled imaging compatible plates in the Luna Crosslinker? Or will the light not get to the ECM?

It is possible to use black-walled plates. However, some optimisation will be necessary. As the black plates limit the amount of light getting to the sample, a higher concentration (2x) of photoinitiator is required, and the crosslinking duration may have to be optimised to achieve the desired cell performance/phenotype. We can help you with the optimisation when you’re ready to get started, but as a general guide we suggest to use 2x concentration of the photoinitiator (dissolve photoinitiator in 500 ul and mix 1:1 with the ECM), and try different crosslinking times, for example, 1, 2, 4, and 8 minutes, and observe if the gel forms and the cell growth.


Can we use black walled imaging compatible plates in the Luna Crosslinker? Or will the light not get to the ECM?

You can certainly do that and it may make it easier to work with. From our tests and experience, however, this is not strictly necessary as the ECM is stable at temperature of up to 50 degrees Celsius.


what ‘low stiffness’ is and what are the final stiffness ranges we can achieve with the Crosslinker? (please specify if this is storage or elastic modulus if you have any ranges).

The stiffness range is approx. 0 - 6 kPa in Compressive/Young's modulus as measured using our standard compression testing regime. Please note that stiffness is, as you know, depending on several factors including sample volume, and should ideally be confirmed in your culture format.

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