LunaGel Photocrosslinkable Extracellular Matrix - 3D Cell Culture Protocol

Follow the six simple steps in this video to create 3D cell culture models:

• Heat LunaGel ECM to 37 °C

• Reconstitute LunaGel photoinitiator in PBS

• Add LunaGel ECM and photoinitator to your cells and gently mix to resuspend cell pellet

• Transfer cell suspension into wells

• Photocrosslink hydrogels in the LunaCrosslinker

• Add cell culture media and incubate

Materials:

• LunaGel™Photocrosslinkable ECM

• LunaCrosslinker™

• phosphate-buffered saline

• cells

• cell culture media

• multiwell tissue culture plates

• micropipettes