Follow the six simple steps in this video to create 3D cell culture models:
Heat LunaGel ECM to 37 °C
Reconstitute LunaGel photoinitiator in PBS
Add LunaGel ECM and photoinitator to your cells and gently mix to resuspend cell pellet
Transfer cell suspension into wells
Photocrosslink hydrogels in the LunaCrosslinker
Add cell culture media and incubate
Materials:
LunaGel™Photocrosslinkable ECM
LunaCrosslinker™
phosphate-buffered saline
cells
cell culture media
multiwell tissue culture plates
micropipettes