LunaGel™ is a photocrosslinkable extracellular matrix (ECM) based on chemically-modified pharmaceutical grade gelatin. The major components of LunaGel™ include the ECM proteins collagen type I, III, IV, and V, as well as connective tissue glycoproteins and proteoglycans. LunaGel™ retains the intrinsic cell-instructive bioactivity of natural ECMs facilitating cell attachment, proliferation, differentiation, migration, and proteolytic degradation. LunaGel™’s unique photocrosslinking technology allows unprecedented control over matrix porosity and stiffness, allowing researchers to replicate the physicochemical properties of a variety of healthy and diseased tissues in 3D cell culture applications.
LunaGel™ ECM has been successfully used in a wide range of applications including cell attachment and proliferation, stem cell culture and differentiation, mechanotransduction assays, cancer spheroid assays, angiogenesis assays, 3D bioprinting, tissue engineering, and more.
LunaGel™ is supplied as a sterile solution with freeze-dried photoinitiator. Available as Low Stiffness (0 - 6.5 kPa) and High Stiffness kit (0 - 25 kPa).
Reconstitute the photoinitiator in PBS, mix with LunaGel™ ECM and cells, and photocrosslink the solution using the Luna Crosslinker™ to form 3D cell culture models. By controlling the duration of light exposure you can produce hydrogels with a specified stiffness. Add your favourite culture media and you're culturing in 3D!
This kit contains enough LunaGel™ to create a total volume of 10 mL hydrogel.
5 mL LunaGel™ ECM (2x solution, sterile)
5 vials of photoinitiator (freeze-dried, sterile)
Low stiffness (0 - 6.5 kPa), 10ml
High stiffness (0 - 25 kPa), 7.5ml
Liquid at temperatures over 4 °C. No heating or cooling required.
Photocrosslinking technology allows highly controlled mechanical properties.
High Bioactivity (cell attachment, proteolytic degradation).
Compatible with automated liquid handling.