top of page

Frequently Asked Questions

For any further questions, contact us at

  • Is LunaGel™ is made of 100% natural ingredients or mixed with any chemical components? The source is this from animal or human or synthetic?
    LunaGel™ contains a proprietary mixture of photocrosslinkable Gelatin and a visible light photoinitiator dissolved in phosphate buffered saline. No chemicals are added to LunaGel™. The product contains chemically modified (porcine skin. cow bone, cold water fish skin) gelatin, hence a semisynthetic product based on animal origin.
  • What are the major components of LunaGel™? Does it contain any biological contaminants?
    The major components of LunaGel™ include the ECM proteins collagen type I, III, IV, and V. Collagens make up approximately 96% of the proteins in the LunaGel™ mixture, the rest is glycoproteins and proteoglycans. The material is free of biological contaminants including free amino acids, nucleotides, and others.
  • For most of the natural gelatins, they would become thermally unstable or melt when temperature is close to 37 degrees. What's the stable temperature window for your porcine gelatin LunaGel™ after curing?
    LunaGel™ forms hydrogels by photocrosslinking of chemically reactive groups and forms stable hydrogels. Unlike unmodified gelatins, LunaGel™ does not melt when heated.
  • Can we use black walled imaging compatible plates in the LunaCrosslinker™?
    It is possible to use black-walled plates. However, some optimisation will be necessary. As the black plates limit the amount of light getting to the sample, a higher concentration (2x) of photoinitiator is required, and the crosslinking duration may have to be optimised to achieve the desired cell performance/phenotype. We can help you with the optimisation when you’re ready to get started, but as a general guide we suggest to use 2x concentration of the photoinitiator (dissolve photoinitiator in 500 ul and mix 1:1 with the ECM), and try different crosslinking times, for example, 1, 2, 4, and 8 minutes, and observe if the gel forms and the cell growth.
  • To avoid repeated warming cycles, would you advise storing the LunaGel™ ECM in single use aliquots?
    You can certainly do that and it may make it easier to work with, however, this is not strictly necessary as the LunaGel™ ECM is stable at temperature of up to 50 degrees Celsius.
  • What is ‘stiffness’ and what are the final stiffness ranges we can achieve with the LunaCrosslinker™?
    For low stiffness kit, the range is 0 - 6 kPa, high stiffness kit allow for up to approx. 25 kPa in Compressive/Young's modulus as measured using our standard compression testing regime. However, the stiffness depend on several factors including sample volume, and should ideally be confirmed in your culture format.
  • What is the recommended number of cells for LunaGel™ system?
    to be added
  • Do I need to keep LunaGel™ ECM in ice?
    No, experiments can be done at room temperature.
  • Why LunaGel™ better than Matrigel?
    Highly tuneable matrix - With LunaGel™, you have the ability to adjust the matrix properties to suit your specific research needs. Consistent properties - Unlike Matrigel, LunaGel™ has consistent properties batch-to-batch, ensuring reproducible results every time. No growth factors - Unlike Matrigel, LunaGel™ does not contain any growth factors, allowing for more control over cell behavior. Work at room temperature - LunaGel™ can be used at room temperature, eliminating the need for costly refrigeration. Readily available - LunaGel™ is readily available, ensuring that your research is never delayed.
  • What is the final polymer concentration in low stiffness porcine skin LunaGel™?
    The final polymer concentration in low stiffness porcine gelatin kits is 5% w/v.
bottom of page